How stem cells contribute to tissues during growth and regeneration remains largely unknown in vertebrates. Skeletal muscle provides an excellent paradigm to investigate this issue since powerful transcription factors that play important roles in specifying and determining skeletal muscle identity have been identified. The Tajbakhsh lab has a wide range of genetically modified mice and has been investigating this question in the context of developmental and regenerative biology.

Project: Mechanisms regulating asymmetric and symmetric cells divisions in adult muscle stem cells in the mouse. This project follows from previous work in the laboratory demonstrating asymmetric segregation ofDNA strands to daughter cells, and direct association of this phenomenon with daughter cell fates (Nature Cell Biology 2006, Nature Reviews Molecular Cellular Biology, 2009; Cell, 2012).

Funding: ERC Advanced Grant. Funding is available for the postdoctoral position for 3 years with the possibility of an extension. Applicants willing to apply for their own independent funding, however, are encouraged to do so.

Requirements: A doctoral degree, strong experience in cellular and molecular biology and microscopy. Proficiency in English is required. Outstanding, highly motivated candidates who are creative minded and independent are encouraged to apply.

Application process: Please send one PDF file to shaht@pasteur.fr with the following:
- cover letter
- concise summary of previous research activities
- curriculum vitae including the publication list and contact details for 2 referees

Prof. Shahragim Tajbakhsh
Stem Cells and Development
Dept. of Developmental & Stem Cell Biology

Pasteur Institute
25 rue du Dr. Roux
75015, Paris, FRANCE
E-mail: shaht@pasteur.fr 

Nous recherchons un(e) ingénieur(e) ou assimilé(e) spécialisé(e) afin de participer à un projet soutenu par l’Association Française contre les Myopathies.

Le projet comporte l'évaluation d'approches de thérapie génique dans un modèle murin de pathologie humaine, l’ataxie épisodique de type 2. Cette maladie neurologique autosomale dominante est due à des mutations dans le canal calcique CaV2.1. L’approche consistera à éteindre l’expression de l’alléle mutée par ARN interférent au niveau cérebrale. L’amélioration du phénotype ataxique sera évaluée par des expériences comportementales. Les compétences recherchées sont l’expérimentation animale chez la souris (chirurgie, comportement) ainsi que des bases en biologie moléculaire et cellulaire.


Contact  : Alexandre Mezghrani, Philippe Lory
alexandre.mezghrani(a)igf.cnrs.fr ; Philippe Lory(a)igf.cnrs.fr
Equipe  :  Canaux calciques  : Structure-Fonction, Régulation et canalopathies (P.Lory)
Institut de Génomique Fonctionnelle
141 rue de la Cardonille
34094 Montpellier Cedex 05 - France

Genethon is launching an International Postdoctoral Program to attract excellent scientists who wish to pursue their career in the field of gene therapy. Postdoctoral research positions are available for a period of three years.

Genethon is a non-profit organization hosting over 200 scientists, with excellent facilities for research, development and manufacturig of gene therapy products. Its main mission is the discovery and clinical development of novel strategies for gene therapy of rare diseases. Genethon’s teams are working on neuromuscular, hematopoietic, liver and skin disorders, on basic mechanisms of virus-host interaction, and on the development of new vector technology (http://www.genethon.fr/en/).

Genethon is located within the Genopole of Evry, France’s leading biotechnology and biotherapy science park in the southern outskirts of Paris.

Philadelphia College of Osteopathic Medicine invites applications for two full-time tenure track faculty positions at the Assistant Professor level to begin July 1, 2013.

 

The successful applicants must have a PhD or equivalent degree and a minimum two years post-doctoral experience, and will be expected to develop a sustainable research program. A research interest related to chronic disorders of aging such as muscular/skeletal degenerative diseases, neurological degenerative diseases, cardiovascular diseases, inflammation, obesity or cancer will be particularly advantageous.The successful applicants will be expected to teach medical students and masters level students in the areas of Physiology, Pharmacology and/or Biochemistry as part of an integrated basic science curriculum.

 

Candidates should submit their curriculum vitae, letter of interest, and names and addresses of three references, preferably from current or former supervisors to:

Philadelphia College of Osteopathic Medicine
Department of Human Resources
4190 City Avenue, Suite 144
Philadelphia, PA 19131
Fax (215) 871-6506
Email: hr(a)pcom.edu

Un poste d’ingénieur ou technicien de laboratoire contractuel (CDD jusqu’à la fin de l’année 2013) est disponible dans l’équipe ‘Myopathies à début précoce’ de l’unité 787 INSERM/UPMC associée à l’Institut de Myologie, Groupe Hospitalier Pitié-Salpêtrière.

 

La personne engagée participera à des recherches multidisciplinaires visant à caractériser les bases moléculaires et physiopathologiques des maladies musculaires héréditaires de l’enfant, et à développer et tester de nouvelles thérapies, notamment pharmacologiques. Une expérience préalable dans un laboratoire de recherche est nécessaire.

 

Les techniques et tâches principales à développer seront les suivantes :
Culture cellulaire : culture de cellules primaires humaines et murines et de lignées murines (C2C12), transfection (siRNA, plasmides), FACS (marquage des cellules pour le tri)
Bactériologie : transformation plasmidique de bactéries compétentes et amplification, purification des plasmides
Biochimie : dosage protéique, Western Blot (chemiluminescence et fluorescence), immunoprécipitation, immunocyto/histochimie
Biologie Moléculaire : extraction d’ADN et d’ARN, RT-PCR, PCR, qPCR, génotypage en routine
Manipulation animale : étude préclinique sur un modèle murin, conditionnement des souris et traitement, montage des muscles, pesée en routine, formation à l'expérimentation animale niveau II
Autres : mise au point et rédaction de protocoles, gestion des stocks et passage de commande

 

Envoyer CV et références à : ana.b.ferreiro(a)gmail.com

 

Project description
Our research work focuses on the mechanisms underlying chromatin dynamics in response to genotoxic stress using cultured mammalian cells as a model system. By combining molecular and cellular approaches with advanced microscopy techniques, our aim is to identify factors involved in regulating chromatin dynamics and their functional relevance for the maintenance of genome and epigenome integrity in response to DNA damage.

We are currently offering two postdoctoral positions to undertake research on the molecular and cellular mechanisms involved in muscle dystrophy caused by mutations in dysferlin. The project has an integrated view of a health problem, and involves interaction with both clinicians and basic scientists.

Successful candidates must hold a PhD degree in the fields of biology or biomedicine, obtained within the last 4 years. Prior knowledge of molecular and cellular biology of skeletal muscle cell is highly desirable, as well as an adequate command of the English language. Good organizational and team work skills are also required.

 

The two positions entail work in the following areas:
1.-­‐ Role of dysferlin, and the impact of dysferlin mutations on DHP receptors and Ca2+ signaling (Drs. Caviedes, Bevilacqua. Univ. of Chile)
The position requires expertise in cell culture, transfection techniques, immunoblotting, use of fluorophores to study intracellular Ca2+ signaling, and patch clamp techniques.

2.-­‐ Role of dysferlin and/or dynamin-­‐2, and the impact of protein mutations, on cortical actin dynamics and vesicle trafficking (Dr. Cárdenas, Univ. of Valparaíso).
The position requires expertise in cell culture, transfection techniques, and fluorescent microscopy techniques. Expertise in TIRF microscopy is highly desirable.

A Postdoctoral position is available for a highly motivated individual in the group of Dr. Fabio Demontis. The lab is located in the Division of Developmental Biology at St. Jude Children’s Research Hospital in Memphis. They use Drosophila and murine models to decipher the conserved mechanisms of muscle aging and proteostasis and how muscle-derived signals (myokines) affect lifespan and the progression of age-related diseases in other tissues (Cell. 2010 Nov 24;143(5): 813-25; Cell Metab. 2012 Jul 3;16(1):97-103; Development. 2009 Mar;136(6): 983-93).

 

In addition to a Ph.D., the candidate should have experience in molecular, biochemical and cell biological techniques. Training in muscle biology, physiology, and/or experience with murine models are preferred but not required.

 

Candidate should have the ability to work independently as well as in collaborative research efforts, and have a strong desire to make a major contribution to the field of aging. The fellow will benefit from both the outstanding research facilities and the highly collaborative St. Jude Community.

 

St. Jude Children’s Research Hospital offers an excellent salary and benefits package and is an equal opportunity employer. Interested applicants should reference job 30936 on all correspondence and apply online at www.stjude.org/AP/position_list. Then send a CV, cover letter describing research experience and interests, and the names and addresses of three references to:

fdemontis(a)genetics.med.harvard.edu